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. Author manuscript; available in PMC: 2017 Apr 13.
Published in final edited form as: Cell Host Microbe. 2016 Apr 13;19(4):529–540. doi: 10.1016/j.chom.2016.03.005

Figure 2.

Figure 2

Cell surface CD3 and CD4 are internalized and CD4 is degraded in SIV infected cells. Tissue stained for A, B CD4 (Green) or E, F CD3 (Green), shows the localization of SIV Env (Red) and Gag (Blue) positive cells below the basement membrane of the vaginal epithelium. A, CD4 and E, CD3 are internalized unlike nearby uninfected cells. B, Example images showing CD4 fluorescence at high (>700 AU, top row), intermediate (400 to 700 AU, middle) and dim (<400 AU, bottom row) levels. Dashed line indicates the border of an infected cell. C, CD4 or G, CD3 fluorescence intensity compared to the spatial distribution, measured as the radius of gyration (RoG) of the CD4 or CD3 signal in SIV positive and negative cells. D, CD4 or H, CD3 RoG in SIV infected cells correlated with fluorescence intensity of SIV Env or DAPI as a control. Scale bars measure 5µm. Animal codes: IN07 and FJ25. See also Figure S2.