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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 15;195(2):621–631. doi: 10.4049/jimmunol.1401823

Figure 3. Treatment of mast cells with sialoL strongly reduces the expression of Il9, Irf4 and Il1b.

Figure 3

Mast cells were stimulated with ionomycin (Iono, 1μM) for 24h in the presence or absence of sialoL (+sialoL, 3.0μM). Isolation of total RNA, library preparation and RNA-Seq was conducted as outlined in the materials and methods section. Transcript counts obtained after alignment were normalized to RPKM (number of reads which map per kilobase of exon model per million mapped reads for each transcript) (A; single experiment). The expression of IRF4 was assessed by western blot analysis in parallel to β–actin as loading control after 24h of stimulation; densitometric quantification was conducted as outlined in the materials and methods section (B). In (A) results of a single experiment is shown. Panel (B) shows one representative from three independent experiments.