| 1 Reaction | ||
| 2 x ddPCR supermix | 12 | |
| Assay mix for NF1 | 1.2 | |
| Assay mix for RPP30 | 1.2 | |
| HaeIII | 0.6 | |
| Subtotal | 15 | Master mixture |
| DNA | 9 | |
| Total | 24 | Final PCR Mixture |
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