Reagent	Stock concentration	Volume	Final concentration
GlutaMAX	200 mM	6 ml	2 mM
2-Mercaptoethanol	10 mM	6 ml	0.1 mM
MEM Non-essential aminoacids (NEAA)	10 mM	6 ml	0.1 mM
Sodium Pyruvate	100 mM	6 ml	1 mM
Pencillin/Streptomycin	10,000 units	3 ml	50 U/ml
FBS		90 ml	15%
CHIR99021*	10 mM		3 µM
PD0325901*	10 mM		1 µM
LIF*	107 units/ml		1000 U/ml
# to prepare ES cell media, add the above components to 500 ml of high glucose DMEM. The ES cell media should not be stored for more than 4 weeks and with inhibitors* not more than 2 weeks.