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. 2016 Apr 11;11:1305–1321. doi: 10.2147/IJN.S92257

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© 2016 Liao et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited.

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(A) Red/green fluorescence intensity for JC-1 staining of MCF-7 cells after treatment of WP1-SeNPs at different concentrations (125, 250, and 500 μg/mL) for 48 hours. (B) WP1-SeNPs induced intracellular ROS level in MCF-7 cells after treatment of WP1-SeNPs at different concentrations (125, 250, and 500 μg/mL) for 48 hours. (C) JC-1 staining images after 48-hour treatments with 125, 250, and 500 μg/mL of WP1-SeNPs. (D) WP1-SeNPs induced ROS generation images under a fluorescence microscope after treatment with WP1-SeNPs at different concentrations (125, 250, and 500 μg/mL) for 48 hours. ^a–dRepresents a significant difference among data in different groups at P<0.05 level.

Abbreviations: DCF, dichlorofluorescein; WP1-SeNPs, walnut peptide 1-selenium nanoparticles; ROS, reactive oxygen species.