Table 2. Index of α-diversity for each DNA extraction method.
Shannon | PowerSoil | Boil | Boil +Tween | QS1 | QS2 | QS3 |
A-1 | 3.38 | 3.47 | 3.45 | 3.61 | 3.56 | 3.38 |
A-2 | 2.84 | 3.44 | 3.04 | 3.37 | 3.31 | 3.05 |
A-3 | 2.92 | 3.40 | 3.21 | NA | NA | NA |
B-1 | 4.13 | 4.04 | 3.91 | NA | NA | NA |
C-1 | 3.39 | 3.55 | 3.52 | NA | NA | NA |
Simpson Index (1-D) | PowerSoil | Boil | Boil +Tween | QS1 | QS2 | QS3 |
A-1 | 0.93 | 0.94 | 0.94 | 0.96 | 0.94 | 0.92 |
A-2 | 0.84 | 0.94 | 0.89 | 0.94 | 0.92 | 0.87 |
A-3 | 0.85 | 0.93 | 0.91 | NA | NA | NA |
B-1 | 0.97 | 0.97 | 0.97 | NA | NA | NA |
C-1 | 0.93 | 0.95 | 0.94 | NA | NA | NA |
Chao-1 Index | PowerSoil | Boil | Boil +Tween | QS1 | QS2 | QS3 |
A-1 | 186 | 180.33 | 169.7 | 183 | 193 | 182 |
A-2 | 174 | 176 | 170 | 183 | 187 | 163 |
A-3 | 196 | 186 | 179 | NA | NA | NA |
B-1 | 203 | 197 | 165 | NA | NA | NA |
C-1 | 181 | 169 | 191 | NA | NA | NA |
>100ppm | PowerSoil | Boil | Boil +Tween | QS1 | QS2 | QS3 |
A-1 | 114 | 119 | 116.3 | 125 | 137 | 114 |
A-2 | 104 | 111 | 100 | 117 | 122 | 101 |
A-3 | 106 | 128 | 114 | NA | NA | NA |
B-1 | 162 | 146 | 139 | NA | NA | NA |
C-1 | 127 | 109 | 122 | NA | NA | NA |
>1000ppm | PowerSoil | Boil | Boil +Tween | QS1 | QS2 | QS3 |
A-1 | 64 | 53.7 | 61 | 65 | 70 | 64 |
A-2 | 53 | 57 | 51 | 57 | 59 | 57 |
A-3 | 60 | 54 | 56 | NA | NA | NA |
B-1 | 97 | 86 | 84 | NA | NA | NA |
C-1 | 64 | 53 | 64 | NA | NA | NA |
A-1: sample ID 1–10 shown in Table 1. A-2: sample ID 11–16. A-3: sample ID 17–19. B-1: sample ID 20–22. C-1: sample ID 23–25. QS1: QIAsymphony. QS2: QIAsymphony + filtration. QS3: QIAsymphony + beads beating + filtration.