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. 2016 Apr 22;11(4):e0154389. doi: 10.1371/journal.pone.0154389

Table 2. Index of α-diversity for each DNA extraction method.

Shannon PowerSoil Boil Boil +Tween QS1 QS2 QS3
A-1 3.38 3.47 3.45 3.61 3.56 3.38
A-2 2.84 3.44 3.04 3.37 3.31 3.05
A-3 2.92 3.40 3.21 NA NA NA
B-1 4.13 4.04 3.91 NA NA NA
C-1 3.39 3.55 3.52 NA NA NA
Simpson Index (1-D) PowerSoil Boil Boil +Tween QS1 QS2 QS3
A-1 0.93 0.94 0.94 0.96 0.94 0.92
A-2 0.84 0.94 0.89 0.94 0.92 0.87
A-3 0.85 0.93 0.91 NA NA NA
B-1 0.97 0.97 0.97 NA NA NA
C-1 0.93 0.95 0.94 NA NA NA
Chao-1 Index PowerSoil Boil Boil +Tween QS1 QS2 QS3
A-1 186 180.33 169.7 183 193 182
A-2 174 176 170 183 187 163
A-3 196 186 179 NA NA NA
B-1 203 197 165 NA NA NA
C-1 181 169 191 NA NA NA
>100ppm PowerSoil Boil Boil +Tween QS1 QS2 QS3
A-1 114 119 116.3 125 137 114
A-2 104 111 100 117 122 101
A-3 106 128 114 NA NA NA
B-1 162 146 139 NA NA NA
C-1 127 109 122 NA NA NA
>1000ppm PowerSoil Boil Boil +Tween QS1 QS2 QS3
A-1 64 53.7 61 65 70 64
A-2 53 57 51 57 59 57
A-3 60 54 56 NA NA NA
B-1 97 86 84 NA NA NA
C-1 64 53 64 NA NA NA

A-1: sample ID 1–10 shown in Table 1. A-2: sample ID 11–16. A-3: sample ID 17–19. B-1: sample ID 20–22. C-1: sample ID 23–25. QS1: QIAsymphony. QS2: QIAsymphony + filtration. QS3: QIAsymphony + beads beating + filtration.