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. 2016 Apr 22;12(4):e1005591. doi: 10.1371/journal.ppat.1005591

Fig 4. TNF mediates an antibacterial effect in macrophages via TNFR1, which is independent of NLRC4 and ROS.

Fig 4

(A-B) WT or knockout BMDM were infected with WT L. pneumophila at MOI 0.1. BMDM were either left untreated (left hand panels) or rTNF was added at the time of infection (right hand panels). 3 days p.i. BMDM were lysed and CFU were quantified on CYE agar plates. (C) WT BMDM were infected with WT L. pneumophila MOI 0.1, with or without the addition of TNFR1-Fc, anti-TNF Ab or anti-IL1β Ab. 3 days p.i. BMDM were lysed and CFU were quantified on CYE agar plates. Data are from 3–7 pooled experiments. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared to WT by Kruskal-Wallis test with Dunn's post test.