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. 2016 Apr 22;11(4):e0154198. doi: 10.1371/journal.pone.0154198

Fig 2. Substrate competition assays.

Fig 2

(A) Phosphorylation of MBP (1.1 μM) and GST-LBRNt(62–92) (0.7 μM) by Akt1 (0.07 μM) in the presence of increasing concentrations of H2B (2.8, 5.6 and 5.6 μM). Upper panel, Coomassie blue staining; lower panel autoradiography. (B) Phosphorylation of H2B (1.4 μM) and GST-LBRNt(62–92) (0.7 μM) by Akt1 (0.07 μM) in the presence of increasing concentrations of R0 peptide (125, 250, 375 and 500 μM). Upper panel, Coomassie blue staining; lower panel autoradiography.