Fig 11. Crossing scheme used to assess the recombination rate between the white-eye (20 cM) and SDL (34 cM) markers on chromosome 1.

The two loci were expected to have a recombination rate of 0.14. Male (M) alleles are dominant in Aedes so that males are M/m heterozygotes and females are m/m homozygous recessive. Wild type males +/+; M/m from a population of interest were crossed to a virgin HWE female (we/we; m/m) to produce F₁ individuals heterozygous for the white-eye locus and the SDL. Male F₁ were we/+; M/m which were backcrossed to virgin HWE females. Subsequent backcross progeny were scored for sex and eye color. Of the four possible phenotypes, white-eye females and wild-type males are parental genotypes, and white-eye males and wild type females are recombinant genotypes.