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. 2004 Jul 7;32(12):3566–3580. doi: 10.1093/nar/gkh677

Figure 6.

Figure 6

Transcriptional activation of the PAcP promoter by TNF-α in PC-3 and HeLa cells. (A) PC-3 cells were transfected with p779 or p1356 promoter constructs. Cells were treated, 24 h after transfection, with 5, 15 or 25 ng/ml of TNF-α for 48 h. Control cells received the solvent alone. CAT assays were performed as described under Materials and Methods. The CAT activity of p779 in control cells was assigned a value of 1.0. The results shown are the average ±SD from two sets of duplicate transfections (n = 2 × 2). (B) HeLa cells were transfected with p779 or p1356 promoter constructs. Cells were treated, 24 h after transfection, with 5, 15 or 25 ng/ml TNF-α for 48 h. Control cells received the solvent alone. CAT assays were performed as described under Materials and Methods. The CAT activity of p779 in control cells was assigned a value of 1.0. The results shown are the average ±SD from two sets of duplicate transfections (n = 2 × 2).