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. 2004 Jul 7;32(12):3566–3580. doi: 10.1093/nar/gkh677

Figure 9.

Figure 9

Effect of TNF-α on PAcP promoter activity. (A) The effects of deletion or mutation in the AGGTGT sequence on TNF-α-activated PAcP promoter activity. The wild-type p1356, ΔAGGTGT and ATGTGT mutant constructs (1 μg of each) were transfected into PC-3 cells. Transfected cells were incubated for 48 h and subsequently were treated with or without 25 ng/ml of TNF-α for 24 h. CAT activities were determined as described under Materials and Methods. Bars represent the SE of triplicates from at least two sets of independent experiments (n ≥ 6). (B) The effect of TNF-α on endogenous PAcP in LNCaP cells. LNCaP cells in tripicates were treated with 5, 10, 25 and 50 ng/ml TNF-α, respectively, for 72 h. Control cells received the solvent alone. The PAcP activity in total cell lysates was determined to represent the PAcP expression. The results shown are the average ± SD (n = 3).