Fig. 3.
The effect of FP on chemokine mRNA stability in BEAS-2B cells. BEAS-2B cells were treated with poly IC (50 μg/ml) in combination with either FP (10−7 M; hatched bars) or DMA (open bars) for 8 h and then harvested immediately (time 0 control) or further treated with actinomycin D for 1,4 or 24 h. RNA was isolated from the cells and CCL5/RANTES (a), CXCL8/IL-8 (b) and CXCL10/ IP-10 (c) mRNA levels were quantified by real-time PCR. The data are presented as percent induction compared with cells collected at time 0 and are the mean ± SEM of 3 independent experiments. * p < 0.05 compared with cells at time 0. n.s. = Not significant.