Fig. 8.
The effect of FP on reporters for NF-κB and IRF-3, and CCL-5 promoter activity in BEAS-2B cells. BEAS-2B cells were transfected with luciferase reporter plasmids and the control vector pRL-TK and 48 h later were incubated with FP (10−7 M; hatched bars) or DMA (open bars) for 30 min and then stimulated in the absence (Cont) or presence of poly IC (50 μg/ml) or TNF-α (10 ng/ ml) for 24 h. Cells were then harvested and subjected to dual firefly and renilla luciferase assays. The relative luciferase activity in cells expressing pNF-κB-Luc (reporter for NF-κB) (a), pISRE-Luc (reporter for IRF-3) (b) and pRANT-WT (CCL5/RANTES promoter) (c) was calculated as fold induction compared with the appropriate control value. The data are the mean ± SEM of 3 independent experiments. * p < 0.05 compared with nonstimulated control cells. ** p < 0.05 compared with DMA-treated cells stimulated with poly IC or TNF-α. Cont = Control; n.s. = not significant.