Fig. 1.

chTNT-3/CpG co-localized with TLR9 in vitro and targeted tumor in vivo. a Fluorescent microscopy demonstrating internalization of chTNT-3/CpG-biotin and CpG-biotin and co-localization with TLR9 (×600 magnification). chTNT-3 was detected using anti-huIgG-AF488 antibodies (top panel, green). CpG-biotin was detected using streptavidin-AF488 (bottom two panels, green). TLR9 was detected using anti-TLR9 and anti-rabbit IgG-AF568 antibodies (red). Images are representative of 3 independent experiments. b Biodistribution of ¹²⁵I-chTNT-3/CpG1826 over 1, 3, and 5 days expressed as  %ID/g. Statistical significances are shown for tumor compared to each of the normal organs. c Biodistribution expressed as tumor/organ ratio of  %ID/g. Statistical significances are shown for comparisons between time points. Error bars represent standard error of the mean (SEM). ***p < 0.001; ****p < 0.0001, n = 5 mice/group