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. Author manuscript; available in PMC: 2017 Mar 26.
Published in final edited form as: Mol Biochem Parasitol. 2016 Mar 26;205(1-2):35–38. doi: 10.1016/j.molbiopara.2016.03.010

Figure 1. Copy number ratio of W marker and Z marker.

Figure 1

A: Square root of the ratio between W marker (W6) and Z marker (Smp_011570.1). Males and females are clearly differentiated from the ratios observed: Females have a high copy number of W marker compared to the Z marker, while in males W markers are absent or rare. B: end point characterization of PCR products. We electrophoresed multiplexed PCR products generated using W and Z marker primers on 2% agarose gels. The size standard is a 100bp ladder (Promega). Females have a 195 bp W6 specific band, and males have a 404 bp Z-specific band. “Double band” individuals are genotypically male but have elevated numbers of W markers relative to most males.