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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Exp Eye Res. 2015 Oct 22;146:370–385. doi: 10.1016/j.exer.2015.09.011

Figure 2. Immunohistochemical markers for the most common cell types in the inner retina correspond to random forest classifications.

Figure 2

Identical fields of cells are shown in each row, with immunofluorescence (IF), H&E staining (H&E), and RetFM-J-recognized cells (Auto-class.) shown in successive columns. (A–C) The retinal ganglion cell marker RBPMS is present in a nucleus auto-classified as RGC (open arrowhead) and absent in a nucleus that has features characteristic of a displaced amacrine cell and was auto-classified as DAC+ (solid arrowhead). (D–F) The astrocyte marker GFAP is present in: a nucleus auto-classified as ND (open arrowhead); an elongated nucleus within a vessel marked by COL4, that has features characteristic of a vascular endothelial cell and was auto-classified as a VEC (solid arrow); and an oval nucleus within a vessel marked by COL4, that has features characteristic of a pericyte and was auto-classified as DAC+ (solid arrowhead). (G–L) The microglial marker IBA1 is present in activated (G–I, open arrowhead) and resting (J-L, solid arrowhead) microglia, both of which were auto-classified as DAC+(M–O) The amacrine marker AP-2α is present in a nucleus auto-classified as DAC+ (closed arrowhead) and absent in a nucleus that has features characteristic of a retinal ganglion cell and was auto-classified as RGC (open arrowhead). C57BL/6J mice at 9–12 weeks of age; scale bar = 10 µm.