Table 5.

Characteristics of frizzled-6 expression and side population cells in six canine primary osteosarcoma cell lines (three tumourigenic and three non-tumourigenic). Frizzled-6 mRNA expression is relative to normal canine osteoblast cell line. All experiments were performed in triplicate and the data is presented as the mean ± SD

Tumourigenic cell lines			Non-tumourigenic cell lines
Cell line	Frizzled-6 expression	Side population	Cell line	Frizzled-6 expression	Side population
UWKOS1	2.406037 ±0.3879	9.90% ±3.46554	UWKOS2	0.001801 ±0.0018	0.20% ± 0.1
UWKOS3	3.582369 ±0.75623	5.93% ± 3.61156	UWKOS6	Not detectable	0.97% ±0.41633
UWKOS7	6.640148 ±4.56758	1.83% ± 1.76163	UWKOS8	0.002455 ±0.00032	3.60% ± 0.96437
Combined†	4.209518 ± 2.99649*	5.89% ± 4.38**	Combined†	0.001419 ± 0.00118*	1.58% ±1.63**

^*p = 0.039, non-tumourigenic cell lines compared with tumourigenic cell lines using a Mann Whitney two-tail test.

^**p = 0.0192, non-tumourigenic cell lines compared with tumourigenic cell lines using a Mann Whitney two-tail test.