Skip to main content
. 2004 Jul 8;32(12):3632–3641. doi: 10.1093/nar/gkh692

Figure 3.

Figure 3

Interaction of recombinant p32 polypeptides with CBF-B in HeLa cells. (A) Two recombinant p32 polypeptides, one corresponding to the full- length p32, mycP32(1–282) and the other corresponding to the processed form of p32 (mycP32) were expressed in HeLa cells. Each polypeptide was expressed as a fusion with the myc epitope tag and was detected by western blot using anti-myc antibody. Cell extracts (mock) without expression of recombinant p32 were used as the control. (B and C) The flag-CBF-B polypeptide was expressed without or together with each recombinant p32 polypeptide in HeLa cells. The cell extracts were immunoprecipitated with an anti-FLAG resin, and then the immunoprecipitate was analyzed by western blot using specific antibodies.