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. 2015 Aug 22;37(1):1357–1368. doi: 10.1007/s13277-015-3928-7

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — The cytoplasmic GalNAc-T3 expression patterns showed specifically positive staining on immunohistochemistry, potentially associated with early recurrence in the ESOSCC patients. Representative images of immunohistochemically strong/weak positivity for GalNAc-T3 in the human ESOSCC samples are shown, demonstrating a cytoplasmic staining pattern in the strong GalNAc-T3-positive cases with postoperative recurrence (case no. 25), compared to the scant cytoplasmic staining pattern in the weak GalNAc-T3-positive cases without any recurrence (case no. 71) (Original magnification: ×100; inset, ×400). In contrast, a cytoplasmic GalNAc-T3 expression was very weakly detectable of adjacent non-carcinomatous squamous epithelium (normal to dysplasia). Each inset provides a representative image of ESOSCC carcinomatous and non-carcinomatous nuclei and cytoplasm on high-power view. H&E hematoxylin and eosin. Bars = 200 μm (×100) and 20 μm (×400)