Fig. 3.

FOXC1 affects viability, colony formation apoptosis and migration in AN3CA and KLE cells. a Western blot was used the detect the expression of FOXC1 when FOXC1 was knocked down. b The cell viability of AN3CA and KLE cells was determined by MTT assay 24, 48 and 72 h after transfection (n = 3,*p < 0.05). c Transwell assay was employed to detect the effect of FOXC1 knockdown on the cell migration of AN3CA and KLE cells (n = 3,*p < 0.05). d Flow cytometry analysis was used to detect cell apoptosis in AN3CA and KLE cells. e Colony formation assay was employed to detect the effect of FOXC1 knockdown on the cell growth activity of AN3CA and KLE cells (n = 3,*p < 0.05)