Table 1.
Antibodies for Flow Cytometry and Immunocytochemistry
| Antibody | Label | Marker expression | Manufacturer | Cat no. | Species | Target species | Diluent |
|---|---|---|---|---|---|---|---|
| CD29 | N/A | β1 integrin | BD Biosciences | 610468 | Mouse | Human | PBS |
| CD34 | PE | Hematopoietic progenitor (HSC) | BD Biosciences | 559369 | Mouse | Dog | PBS |
| CD44 | FITC | Hyaluronic acid receptor | eBiosciences | 115440 | Mouse | Dog | PBS |
| CD90 | PE | Thy-1, fibroblasts, MSC, HSC | eBiosciences | 125900 | Mouse | Dog | PBS |
| Goat anti-mouse IgG | FITC | Secondary antibody | Sigma-Aldrich | F9006 | Goat | Mouse | PBS |
| β-actin | N/A | Cytoskeletal protein | Thermo Scientific | RB-9421-P0 | Rabbit | Dog | TBS |
| Sox-2 | N/A | Transcription factor | LifeSpan BioSciences | LS-B4562 | Rabbit | Dog | 5 % Skim milk/PBS |
| PPAR-γ | N/A | NHR/NR1 Thyroid homone-like | LifeSpan BioSciences | LS-B651 | Rabbit | Dog | 5 % BSA/TBS |
| OPN | N/A | Secreted Phosphoprotein 1 | LifeSpan BioSciences | LS-B425 | Rabbit | Dog | 5 % BSA/TBS |
| CD29 | N/A | β1 integrin | BD Biosciences | 610468 | Mouse | Human | TBS |
| CD44 | N/A | Hyaluronic acid receptor | Monoclonal Antibody Center | DG-BOV2037 | Mouse | Dog | TBS |
| Goat anti-rabbit IgG | HRP | Secondary antibody | Santa Cruz Biotechnology | sc-2004 | Goat | Rabbit | TBS |
| Goat anti-mouse IgG | HRP | Secondary antibody | Santa Cruz Biotechnology | sc-2005 | Goat | Mouse | TBS |