Western blot analysis of SPM proteins obtained from young (4 months) and old (15 months) mice raised with DHA-adequate diet. Quantitation was based on the band intensity normalized to cadherin using three biological replicates. The data are expressed as mean ± standard deviation (n=3–4). Statistical analysis was performed using Student’s t-tests. *, p< 0.05; **, p< 0.01. 4M; 4 month-old, 15M; 15 month-old, Ade, DHA-adequate.