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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Neurobiol Aging. 2016 Feb 16;41:73–85. doi: 10.1016/j.neurobiolaging.2016.02.007

Fig. 6.

Fig. 6

Effect of raising brain DHA content on synaptic protein level and cognitive function in aged mice. a, Timeline of the diet. Mice were fed with DHA-adequate (Ade) or DHA-deficient diet (Def) diet for 17 months, or fed with the deficient diet for 15 months followed by the adequate diet for 2 months to increase the brain DHA level. b–c, Brain DHA (b) and synaptic protein levels (c) at 17 months of age after feeding different diets. d, Effect of brain DHA status on cognitive function evaluated by novel objective recognition test. The 17-month DHA-deficient mice showed significantly shorter novel object exploration as compared to the adequate group, indicating impaired recognition memory. This difference in the novel object recognition performance disappeared when 15 month old DHA-deficient mice were placed on the adequate diet for 2 months. Student’s paired t-test was used to assess statistical differences. Data are presented as mean ± standard deviation (in b,c; n=3–4) or SEM (in d; n=6–8). ***, p < 0.001; **, p < 0.01; *, p < 0.05.