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. 2004 Jul 7;32(12):e98. doi: 10.1093/nar/gnh094

Figure 6.

Figure 6

(A) DNA blocks amplified using 60 or 90 nt oligonucleotides separately using the PTDS method. M is DNA marker DL2000. Lane 1: 460 bp block; lanes 2–5: four 500 bp blocks; lane 6: 750 bp block; lanes 7 and 8: two 880 bp blocks. (B) A full-length vip3aI gene amplified using the PTDS method. M is λ-DNA Hind III marker. Lane 1: a full-length vip3aI gene amplified from three blocks that were derived from 90 nt oligonucleotides; lane 2: a full-length vip3aI gene amplified from five blocks that were derived from 60 nt oligonucleotides. (C) A full-length vip3aI gene amplified using single-step overlap extension PCR. M is λ-DNA Hind III marker. Lane 1: a full-length vip3aI gene amplified using 60 nt oligonucleotides by single-step successive PCR; lane 2: a full-length vip3aI gene amplified using 90 nt oligonucleotides by single-step overlap extension PCR; lanes 3 and 4: full-length amplified vip3aI gene fragments using outermost primers.