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. 2016 Apr 23;17:301. doi: 10.1186/s12864-016-2626-1

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© Vicente et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Colony PCR results indicating that group II intron-based vectors successfully targeted the Serratia sp. LCN16 oxyR and kat genes. a Introns were inserted in the position 614|615 of oxyR gene, oxyR::int(614); and in the position 808|809 of katA gene, katA::int(808). For both genes, the arrows indicate the position of forward and reverse primers designed to infer intron insertion. b L1 and L4 indicate 100-bp and 1-kb molecular markers, respectively. L2 and L5 correspond to the oxyR (846 bp) and katA (292 bp) fragments. L3 and L6 correspond to mobile group II intron integrated in oxyR (about 3Kb) and katA (about 2-3Kb), respectively