Table 4.

List of strains, plasmids and primers used in the present study

Strain, plasmid and primers	Genotype or phenotype
Serratia sp.
LCN16 WT	WT resistant to ampicillin and erythromycin.
LCN16 oxyR::int(614)	Knockout mutant of oxyR with group II intron inserted in the position 614; Resistant to gentamicin and kanamycin.
LCN16 katA::int(808)	Knockout mutant of katA with group II intron inserted in the position 808; Resistant to gentamicin and kanamycin.
Escherichia coli
DH5α	Competent cells (Sigma-Albrich)
Plasmids
pAR1219	TargeTron vector with chloramphenicol resistant
pAR1219-ΩGm	Constructed vector with gentamicin resistant gene aacC
pACD4K-C	TargeTron vector; kanamicin RAM marker (for chromosomal insertion) and chloramphenicol resistance (plasmid propagation)
pACD4K-C_oxyR	TargeTron vector with intron RNA retarget for oxyR gene
pACD4K-C_kat	TargeTron vector with intron RNA retarget for kat gene
pBK-miniTn7-ΩGm	Tn7 plasmid constructed with gentamicin resistant gene aacC
PCR Primers (5′-3′)
aacC1_IfFor	CATACTCTTCCTTTTTCAATATTATTG
aacC1_IfRev	TAACTGTCAGACCAAGTTTACTC
oxyR_614|615 s-IBS	AAAAAAGCTTATAATTATCCTTAGGAAGCTGGTCAGTGCGCCCAGATAGGGTG
oxyR_614|615 s-EBS1d	CAGATTGTACAAATGTGGTGATAACAGATAAGTCTGGTCACTTAACTTACCTTTCTTTGT
oxyR_614|615 s-EBS2	TGAACGCAAGTTTCTAATTTCGATTCTTCCTCGATAGAGGAAAGTGTCT
oxyR_KO_chkFor	CGTGGTCTGGAGGGAAACAA
oxyR_KO_chkRev	CATAACGACTGCGCAATGGG
katA_808|809a -IBS	AAAAAAGCTTATAATTATCCTTATAATCCGGATTTGTGCGCCCAGATAGGGTG
katA_808|809a EBS1d	CAGATTGTACAAATGTGGTGATAACAGATAAGTCGGATTTGCTAACTTACCTTTCTTTGT
katA_808|809a -EBS2	TGAACGCAAGTTTCTAATTTCGGTTGATTATCGATAGAGGAAAGTGTCT
katA_KO_chkFor	GGTGAAGTTCCATTTCCGCTGC
katA_KO_chkRev	GGGTTCACCGCTACCTGTTCAAC
RT-qPCR primers (5′-3′)
gyrA_For	TTATCTCCCTGATTGTGCCA
gyrA_Rev	CATTACGCTCGCTCACCTTA
oxyR_For	TTTAGAGTACCTGGTCGCCTTG
oxyF_Rev	ATCACACCCAGTTCGTCTTCC
katA_For	CCAGATTATGCCTGAACACG
katA_Rev	TGCAGTTCGAAGAAACCAAC
katG_For	AGCGGTAAGCCAAATACACC
katG_Rev	AATCGAAGTCAGGGTCCATC