Figure 2.

Restriction analysis of ligation products. (A) Digestion of un-nicked homoduplex and heteroduplex plasmids with NcoI. The ligation products was digested with NcoI endonuclease and then electrophoresed in 1% agarose gel. The homoduplex plasmid was digested into three fragments of 2.3, 1.9 and 317 bp. The ethidium bromide staining of the 317 bp fragment was too weak to be photographed. A faint band at 2.6 kb is likely the incompletely digested product that generates 2.3 and 317 bp fragments. The heteroduplex plasmid was digested into two fragments of 2.6 and 1.9 kb. (B) Digestion of nicked homoduplex and heteroduplex plasmids with NcoI. Homoduplex was digested into three fragments of 3.3, 1.0 and 317 bp, whereas the heteroduplex was digested into two fragments of 3.3 and 1.3 kb.