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. 2016 Mar;12(1):25–35.

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© Debipriya Banerjee et al. Licensee Master Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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HPLC assessment of arachidonic acid release from isolated platelet membrane preparation treated with exogenous Ca²⁺. Platelet membrane preparation (2 mg) was treated with 1nM extraneous Ca²⁺ (CaCl₂). The fatty acids were extracted and chromatographed in HPLC. For comparison, pure sample of arachidonic acid (Sigma) was used as standard as shown. “Sample” in the figure means isolated platelet membrane preparations after exogenous calcium incubation and “Standard” means characteristic HPLC peak for pure arachidonic acid. The sample absorbance was found to be statistically significant where P<0.01 determined by Student t-test. The results shown here is a typical representative of 7 different experiments using washed PRP from 7 different volunteers.