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. 2004 Jul 12;32(12):e103. doi: 10.1093/nar/gnh101

Figure 3.

Figure 3

The influence of NaCl on the dbpr dependence of SG/dsDNA differential absorption values (A) and fluorescence (B). (A) Differential absorption values at 494 nm of SG/λDNA complexes at increasing dbprs in TE buffer pH 7.5 that did not contain NaCl (circle) and in TES buffer pH 7.5 that contained 100 mM NaCl (solid square). (B) Samples containing 0.15 μM bp of λDNA were titrated with increasing amounts of SG ranging from 0 to 1.5 μM in the presence of 0 mM (solid squares), 25 mM (solid circles), 50 mM (solid triangles, up) and 300 mM (solid triangles, down) NaCl.