Table 2.
The contents of five major compounds in HFBP by HPLC analysis.
| Peak number | t R (min) | Compound name | Linearity | Sensitivity (µg/mL) | Precision (RSD%, n = 6) | Stability (RSD%, n = 3) | Content (%) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Equation | R 2 | LOD | LOQ | Intra-day | Inter-day | |||||
| 1 | 16.37 | Prim-O-glucosylcimifugin | y = 40565x + 149.55 | 0.9998 | 0.03 | 0.05 | 0.24 | 1.38 | 0.40 | 0.202 |
| 2 | 19.23 | Calycosin-7-O-β-D-glucoside | y = 73499x + 64.019 | 0.9997 | 0.027 | 0.055 | 0.26 | 1.58 | 0.51 | 0.043 |
| 3 | 21.33 | Macrotin | y = 60171x + 50.145 | 0.9997 | 0.047 | 0.023 | 0.16 | 1.59 | 0.56 | 0.035 |
| 4 | 24.03 | 4′-O-β-D-Glucosyl-5-O-methylvisamminol | y = 45658x + 179.85 | 0.9996 | 0.031 | 0.051 | 0.17 | 1.33 | 0.18 | 0.221 |
| 5 | 29.69 | Ononin | y = 76593x + 60.616 | 0.9997 | 0.20 | 1.40 | 0.48 | 0.021 | ||