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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Protein Expr Purif. 2016 Feb 23;122:45–55. doi: 10.1016/j.pep.2016.02.014

Fig. 6.

Fig. 6

Recombinant hSEAP production. Sf-RVN and Sf9 cells were infected with an Sf-rhabdovirus-negative stock of AcP(−)p6.9hSEAP at an MOI of (A, B, C) 0.1 or (D, E, F) 5 pfu/cell. Triplicate samples were harvested at various times post-infection, cell-free media were prepared and assayed for (A, D) hSEAP activity, as described in Materials and methods, and the average results were plotted with error bars representing the confidence intervals (P<0.05). One set of cell-free media was also used to measure (B, E) total extracellular hSEAP production levels by immunoblotting analysis, with (C, F) scanning laser densitometry to estimate relative immunoreactive band intensities, as described in Materials and methods.