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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Protein Expr Purif. 2016 Feb 23;122:45–55. doi: 10.1016/j.pep.2016.02.014

Fig. 7.

Fig. 7

Recombinant hEPO production. Sf-RVN and Sf9 cells were infected with an Sf-rhabdovirus-negative stock of AcP(−)p6.9hEPO at an MOI of 5 pfu/cell. Samples were harvested at various times post-infection and cell-free media were prepared and assayed for (A) total extracellular hEPO production levels by immunoblotting analysis, with (B) scanning laser densitometry used to estimate relative immunoreactive band intensities, as described in Materials and methods.