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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Protein Expr Purif. 2016 Feb 23;122:56–63. doi: 10.1016/j.pep.2016.02.010

Figure 4. NMR spectra of BCL-XL and BCL-XL(Ct) in lipid nanodiscs, mixed lipid-DePC, or DePC micelles.

Figure 4

(A–C) Solution NMR 1H/15N correlation spectra of BCL-XL in nanodiscs (red), BCL-XL in 170 mM DePC (black), BCL-XL(Ct) in nanodiscs (blue) or BCL-XL(Ct) in nanodiscs supplemented with 170 mM DePC (mixed lipid-DePC; orange). (D) Secondary structure and dynamics features of BCL-XL(Ct) in mixed lipid-DePC. The HA chemical shift difference (ΔHA) was calculated by subtracting the random coil values from the experimental chemical shifts. Experimental 1H-1H NOE data were obtained between atomic sites: HN(i) and HN(i+1), HA(i) and HN(i+1), or HA(i) and HN(i+3). HN Peak intensities (Io) were derived from the 1H/15N correlation spectra. (E) One-dimensional 1H spectra of 15N labeled BCL-XL(Ct) in mixed lipid-DePC obtained in H2O or 2H2O.