Fig. 9.

Promoter activities in the 3′-region of ntrC and upstream of blr1853 under different conditions. a The 3′-region of ntrC with two convergent TSSs without mapped promoters: Bja_TSS_8751 of the sRNA 3′-seR and Bja_TSS_8753 of the asRNA 3′-asR (designated no. 11, and 12 in Fig. 5). b The promoter activities of the regions upstream of the two TSSs shown in a were measured in the exponential and stationary growth phases. c The blr1853 locus with three TSSs: Bja_TSS_3937 preceded P_cyp, Bja_TSS3938 preceded by P_int and Bja_TSS_3939 preceded by P_as (the respective numbers of these TSSs in Fig. 5 are no. 10, 15 and 13). d The activities of the promoters P_cyp and P_int were measured in aerobic cultures grown to the exponential and stationary growth phase, and in exponentially growing cultures under microoxic conditions. For the analysis of P_as, see Fig. 4d. a and c show cDNA reads, mapped TSSs (red flexed arrows), mapped promoters (red filled rectangles), TSS upstream regions with promoter activities lacking mapped promoters (red empty rectangles), a mapped terminator (dark grey rectangle marked with T), mRNAs (bars with differently colored regions – light grey and red depicting annotated ORFs and UTRs, respectively) and new transcripts (red bars representing internal sense RNAs or as RNAs). b and d show the results from beta-galactosidase measurements in B. japonicum. Respective 200 nt TSS upstream regions were cloned in front of the lac-operon. Shown are results from three independent experiments with technical duplicates and error bars depicting the standard deviation