Fig. 5.

C-A1 reduces accumulation of capsid into the nucleus of infected cells. a Jurkat cells were infected at an MOI of 0.5 with HIV-1_GFP WT, A105S or N74D mutants in the presence or absence of 3 μM C-A1. Cells were fractionated 16 h post-infection and the distribution of capsid, Na/K ATPase and DNA Topoisomerase II (Topo II) in the nucleus and cytoplasm examined by Western blot. b The ratio of cytoplasmic versus nuclear capsid was calculated using ImageJ and normalized for Na/K ATPase (cytoplasmic fraction) or TopoII (nuclear fraction). Average values ±SD of at least four independent experiments are shown for WT virus and three experiments for N74D or A105S viruses. c Fold change in the cytoplasmic/nuclear ratio of capsid based on ImageJ quantification. Statistical significance was calculated using Student’s unpaired, two-tailed t-test