Fig. 7.

C-A1 enhances capsid binding to CPSF6. a Cellular extracts expressing CPSF6-FLAG were incubated with in vitro assembled HIV-1 CA–NC complexes at room temperature for 1 h in the presence of C-A1 or PF74. The mixtures were applied onto a 70 % sucrose cushion and centrifuged. INPUT represents the mixtures before being applied to the 70 % cushion. The INPUT was Western blotted using anti-FLAG antibodies. The pellet from the 70 % cushion (BOUND) was analyzed by Western blotting using anti-FLAG and anti-capsid antibodies. The results of three experiments were similar and standard deviations are shown. b Similar binding experiments were performed using increasing concentrations of C-A1. The results of three experiments were similar