Fig. 4.

IL-1β release is reduced in LLOME and particle treated ctss ^−/− macrophages. a ELISA analysis of secreted IL-1β levels in supernatants from wildtype and ctss ^−/− i) LPS-primed peritoneal macrophages and ii) LPS-primed BMDMs which were treated for 16 h with LLOME (0.5 mM), n = 4 +/− standard error. iii) ELISA analysis of secreted IL-1β levels in supernatants from wildtype and ctss ^−/− BMDMs which were primed with LPS (100 ng/mL) prior to stimulation with LLOME (0.5 mM). Supernatants were harvested at timed intervals from 0.5 h to 4 h, n = 3 +/− standard error. b ELISA analysis of secreted IL-1β levels in supernatants from wildtype and ctss ^−/− LPS-primed peritoneal macrophages which were treated for 16 h with a concentration range of i) alum, ii) silica, iii) PSNP, n = 4 +/− standard error. c ELISA analysis of secreted IL-1β levels in supernatants from wildtype and ctss ^−/− LPS-primed BMDMs which were treated for 16 h with a concentration range of i) alum, ii) silica, iii) PSNP, n = 4 +/− standard error