(A) RNA in situ hybridization experiments in the nodose/jugular complex revealed that P2ry1, Npy2r, and Gpr65 are expressed in subsets of vagal sensory neurons.
(B) Two color in situ hybridization experiments for indicated genes revealed largely non-overlapping neuron populations. The numbers of cells expressing one receptor (red or green) or both receptors (yellow) were counted.
(C) The indicated Cre lines were crossed with lox-L10-GFP mice, and in offspring, fixed cryosections of the nodose/jugular complex were imaged by fluorescence microscopy. Native GFP fluorescence (green) and a fluorescent Nissl stain (gray) were visualized. Scale bars, 100 mm. See also Figures S1 and S2.