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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Ocul Surf. 2016 Jan 22;14(2):113–120. doi: 10.1016/j.jtos.2015.12.006

Figure 1.

Figure 1

Expression of stem cell genes by CSSCs. CSSCs from digests of human limbal stroma were expanded in a low serum-culture medium that maintains their stem cell phenotype, and the relative expression of mRNA from genes identified in stem cells was determined by RT-PCR as described by Basu et al.31 The first group represents genes expressed in pluripotent cells (SSEA4, OCT4, SOX2, KLF4, NANOG, REX1). The second group represents genes expressed by MSC (BMI1, CXCR4, ABCG2). The third group represents markers of neural stem and progenitor cells (NESTIN, CDH2, NGFR). The final group (PAX6, SIX6) are expressed by ocular precursors in embryonic development. Delta Ct in the horizontal axis estimates the difference in abundance between the mRNA in question and 18S RNA as a power of 2.