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. 2016 Apr 11;2016:7369491. doi: 10.1155/2016/7369491

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Copyright © 2016 Chuanhai Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EN1 expression during brown adipogenesis. (a-b) Primary brown adipocyte (SCA1+/CD31−/CD11b−) was isolated from fetal C57BL/6J mouse BAT and (c) the mRNA and (d) protein expression of EN1 were analyzed during brown adipogenesis. Relative levels of EN1 protein were calculated based on densitometry analysis (bottom panel of (d)). Data were analyzed by unpaired Student's t-test in (c). ^∗ p < 0.05, ^∗∗ p < 0.01 versus control or analyzed by one-way ANOVA with Tukey's post hoc test; n = 3–5 per group.