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Overexpression of EN1 stimulates brown adipogenesis. Lentiviral mediated overexpression of EN1 was confirmed by (a) RT-PCR or (b) western blot analysis. Brown adipogenesis was assessed by (c) phase contrast microscopy and Oil-Red O staining. (d, e) Adipogenic and thermogenic gene expression and (f) BAT related protein expressions were analyzed during brown adipogenesis. Data are mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, n = 3.
