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. 2016 Apr 25;10:169. doi: 10.3389/fnins.2016.00169

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Copyright © 2016 Voziyanov, Kemp, Dressel, Ponder and Murray.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Probe holder with custom grips for surgically attaching head plate. The head plate is held by 3D printed head plate grips that are attached to the end of a standard probe holder for a stereotaxic device (see Supplementary Material). (A) Probe holder with custom grips attached. (B) View showing details of grips. (C) Grips in use during surgery to attach a head plate. The cut-out in the head plate around the craniotomy provides room to attach an injury hub for a fluid percussion injury, however, a glass cover slip can be attached to this area for use as a cranial window, or it can be used for applying additional adhesive, if desired. For our experiments, a GRIN lens is attached to a round glass cover slip that is glued under the opening that is just rostral and to the left of the craniotomy. The lens is lowered into a separate craniotomy (not visible in this photo) as the head plate is lowered.