Figure 9.

In vivo image set of astrocytes in lower levels of cortex using an implanted GRIN lens. A z-stack of 51 images using 1 μm steps was acquired using a GRIN lens implanted in an adult GFAP-GFP mouse. Astrocyte cell bodies and processes were observed throughout the volume. Z-projections were made of several consecutive image planes (steps). All images were projections of five consecutive image planes with the exception of Image E in which 10 planes were used for the projection. The most dorsal image plane was defined as plane one and the most ventral image was plane 51. Z-projection planes for (A–H) were, as follows: (A) 1–5, (B) 5–10, (C) 10–15, (D) 15–20, (E) 25–35, (F) 35–40, (G) 40–45, (H) 45–50. A Gaussian filter with σ = 1.33 was applied to all channels to reduce background noise. Scale bar denotes 5 μm for all images.