Table 1.
Primer sequences used for Quantitative real-time-PCR analysis.
| Seq-ID | Gene name | Primer Sequence (5′-3′) and Tm(°C) | GO-Cellular Component |
|---|---|---|---|
| AJ585201 | Actin | F TCCTCACGCCATTCTT (59.10) R TCTCCTTGATGTCCCT (59.08) |
GO:0008372; cellular component |
| Unigene42814 | Sterol 24-C-methyltransferase | F CGAACTTCAAGCACACTCGT (59.09) R CAGTACCCTTTGGTGCAATG (59.05) |
GO:0009506//plasmodesma;GO:0005773//vacuole;GO:0005783//endoplasmic reticulum |
| Unigene42248 | GDP-D-mannose 3′, 5′-epimerase | F AATCCAACCATTCGGTCATT (59.10) R TACAAGCCGATGAGGCATAG (58.90) |
– |
| Unigene19563 | Acyl-CoA dehydrogenase | F GCAACCAACATTGAAACGAG (59.17) R TGATGACGAGGCTTAGTTGG (58.87) |
– |
| CL1489 | Predicted protein | F GCTATTACCGCAAGGTCCAT (59.07) R TCGATCATATGGCACAACAA (58.49) |
– |
| CL11729 | Abscisic acid receptor PYR/PYL family |
F GCTGCACTTCACCAAAGAAA (59.05) R TGCTCTCGAATTTCTCAACG (59.15) |
– |
| CL10698 | Hexokinase | F TGGACCTAGGAGGGACAAAC (58.99) R AACCAAACAATTCCGAGGAG (59.03) |
GO:0005739//mitochondrion;GO:0009536//plastid;GO:0005773//vacuole;GO:0005634//nucleus;GO:0005886//plasma membrane |
| Unigene30003 | Hypothetical protein CHLNCDRAFT_56419 |
F TCCGCAAACAAATCGTAGAG (58.92) R CAAAGACAGGACCAGCAGAA (59.01) |
– |
| CL12576 | 3β-hydroxysteroid-dehydrogenase | F AAGCATACACGCAAACGAAG (58.99) R CAAGTGCGCAGCTCATTTAT (59.09) |
GO:0016020//membrane |
| Unigene48238 | Uncharacterized protein LOC100501669 |
F GAAGAGGTATGCTGATGGCA (58.83) R AGACCATACGCCTTGGTAGC (59.22) |
GO:0005739//mitochondrion;GO:0009579//thylakoid;GO:0009507//chloroplast;GO:0016020//membrane;GO:0005783//endoplasmic reticulum |
| Unigene2564 | Hypothetical protein PHYSODRAFT_484706 |
F GCCGATCCAACTCATACCTT (59.01) R TGCATGCTTGAGGATACCAT (59.10) |
GO:0009941//chloroplast velope;O:0048046//apoplast;GO:0010319//stromule;GO:0005829//cytosol;GO:0009570//chloroplaststroma;GO:0009579//thylakoid |
| Unigene3201 | Shaggy-related protein kinase | F CTCGTAACACCGACACCATC (59.01) R CGTTGTCTAACATCCATCGG (59.00) |
GO:0005829//cytosol |
| Unigene28957 | Predicted protein | F ACGACCAAAGTGGTGAACAA (59.03) R TGCCGAAATCAAGACACAAT (59.13) |
GO:0009941//chloroplast envelope;GO:0005794//Golgi apparatus;GO:0005783//endoplasmic reticulum |
| Unigene41363 | Predicted protein | F CGACTACAAGGACTGGCAGA (59.03) R CAATACCACCCACAGCTTCA (59.57) |
– |
| Unigene21403 | Aspartate aminotransferase | F GGGATGCATTTGGAGATGA (59.39) R TGAGCAGAGTCGTTGCTTCT (58.91) |
GO:0009507//chloroplast |
| CL7857 | Os08g0127100 | F TTCAGCCTCTCATGGTTCAC (58.80) R GCCACGATCATCAGAATCAC (59.04) |
GO:0016021//integral to membrane; GO:0005886//plasma membrane |