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. 2016 Mar 23;173(10):1589–1601. doi: 10.1111/bph.13460

Figure 2.

Figure 2

SIRT1 shRNA treatment up‐regulated Col1, Col3 and α‐SMA expression in hypertrophic scar fibroblasts. (A) The effects of SIRT1 shRNA on SIRT1, Col1, Col3 and α‐SMA mRNA levels in fibroblasts derived from hypertrophic scars were analysed by real‐time PCR and normalized to GAPDH (n = 6). (B) Representative western blot analysis for SIRT1, Col1, Col3 and α‐SMA protein levels in hypertrophic scar‐derived fibroblasts after SIRT1 down‐regulation by shRNA treatment (n = 6). The results represent the mean ± SEM of six independent experiments. *P < 0.05.