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. 2016 Apr 25;6:24979. doi: 10.1038/srep24979

Figure 2. Single-turnover kinetics of mouse and human EndoV on ss and ds IIUI-RNA substrates.

Figure 2

30 nM enzymes were incubated with 1 nM ss IIUI-RNA or ds IIUI-RNA substrates and samples were withdrawn at time points as indicated and analyzed by PAGE. A one phase association model (solid line) was fitted to three parallel, independent experiments for calculation of the turnover rate kobs.