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. 2016 Apr 25;6:24979. doi: 10.1038/srep24979

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Copyright © 2016, Macmillan Publishers Limited

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Bandshift assays with wild type or mutant D52A mEndoV (0.25–1 μM) with ss/ds IIUI-RNA, ss/ds I-RNA and ss/ds dIrG-DNA substrates were performed. Human EndoV (0.25 μM) was included in all assays. After incubation on ice for 15 minutes, bound RNA/DNA (=B) were separated from free substrates (=F) by 10% native PAGE.