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. 2016 Apr 25;6:24704. doi: 10.1038/srep24704

Figure 5. Suppression of NOTCH signaling inhibits chemoresistance in CAL27 cell line.

Figure 5

(A) Representative flow cytometry photos shows DAPT treatment attenuate chemotherapy agent Docetaxel (DTX, 10 μM), cisplatin (CDDP, 10 μM), 5-fluorouracil (5-FU, 15 μM) induced CD44+ CD133+ CAL27 cell population. Experiment repeated twice. (B) Representative tumor sphere formation of combined DAPT treatment with chemotherapeutic agents; Scale bar, 50 μm. (C) Quantification of in vitro sphere number and size of DTX, CDDP, 5-FU treated CAL27 with or without DAPT. (D) Representative flow cytometry shows side population changes of DTX, CDDP, 5-FU treated CAL27 cells with or without DAPT. Stained with Hoechst 33342 and excited by UV light; Hoechst Red, 675 nm channel; Hoechst Blue, 450 nm channel. (E) Quantification of side populations from triple experiments; Data present as mean ± SEM, **P < 0.01; ***P < 0.001.