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. 2016 Apr 25;6:24940. doi: 10.1038/srep24940

Figure 5. Effect of kaempferol on the ERK pathway and Sp1phosphorylation in HepG2 cells.

Figure 5

HepG2 cells were treated with kaempferol (100 μM) for the indicated times in the presence or absence of U0126 (10 μM). Whole cell extracts were subjected to SDS–PAGE and immunoblotting (IB) with anti-p-ERK, anti-ERK, anti-p-Sp1(Thr-453), anti-p-Sp1(Thr-739), anti-Sp1, or anti-β-actin antibodies. (a) The signals (n = 3) were quantified and normalized by β-actin signals, and the signals of the control group were represented as one. (b) The signals were quantified and normalized by β-actin signals, and the signals of the control group were represented as one. Similar results were obtained in two separate experiments. (c,d) Similar results were obtained in two separate experiments.