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. 2016 Apr 25;6:24940. doi: 10.1038/srep24940

Figure 7. Effects of other flavonoids on LDLR gene expression and ERK pathway.

Figure 7

HepG2 cells were cultured with the indicated flavonoids (100 μM) for 24 h, and total RNA and whole cell extracts were isolated. (a) Real-time PCR analysis was performed. mRNA levels were normalized to those of GAPDH mRNA and were expressed relative to those in the vehicle (DMSO)-treated controls. All data are expressed as mean ± SEM, n = 3. *P < 0.05. (b) Whole cell extracts were subjected to SDS-PAGE and immunoblotting (IB) with anti-p-ERK, anti-ERK, or anti-β-actin antibodies. Similar results were obtained in two separate experiments.